天行app怎么用

Harnessing RNA Regulation to Direct Protein Evolution and Control Mammalian Gene Expression

Dr. Bryan Dickinson | University of Chicago

怎么修改手机ip地址?手机ip地址更改方法介绍_手机 ...-脚本之家:2021-9-11 · 一般手机连接wifi后都是使用DHCP自动获取ip，那么如果获取不了或者我们想固定ip方便管理，如何操作呢？这个时候我们就需要更改ip地址。那么怎么修改手机ip地址?这里详细地教大家手机ip地址更改方法，希望对大家有所帮助

Harnessing RNA Regulation to Direct Protein Evolution and Control Mammalian Gene Expression

I will present two recent technologies our group has developed that harness RNA regulation – one for basic science purposes and one for therapeutic development. First, I will describe new methods that use our RNA polymerase-based biosensors to harness evolution in order to probe the emergence of “selectivity” between biomolecular interfaces, in particular, protein-protein interactions (PPIs). Using a combination of high-throughput biochemical methods, ancestral reconstruction, and a new rapid evolution technology, we developed a model system involving the BCL-2 family of apoptotic regulatory proteins to probe fundamental evolutionary questions about PPIs and how selectivity (or not) emerges between them. In the second half of the talk, I will discuss therapeutic opportunities involving RNA regulation and “epitranscriptomics”. While RNA regulation offers exciting opportunities to create genetic therapies that are reversible and tunable, most current approaches rely on large, microbially-derived systems that pose clinical challenges. We developed the CRISPR/Cas-inspired RNA targeting system (CIRTS), a new protein engineering strategy for constructing programmable RNA regulatory systems entirely from human protein parts. The small size and human-derived nature of CIRTS provides a less-perturbative method for fundamental studies as well as a potential strategy to avoid immune issues when applied to epitranscriptomic therapies.